You are here

Custom allele conversion

The KOMP cloning strategy has employed a gene trapping strategy using promoter-driven and promoter-less cassettes to generate knockout-first alleles.  In each case, acritical exon, common to all transcripts of a particular gene is targeted.  This critical exon(s) when deleted causes a frame shift mutation that produces a null allele (Ref Ryder 2013, Mamm Genome 24:286-294; Coleman et at (2015) Frontiers in Endocrinology doi: 10.3389/fendo.2015.00105)

An overview of the different alleles is shown below;