Cre driver activity profiling
We offer services in the characterisation of cre driver activity, at both adult and embryonic stages. Cre driver mouse strains are mated with lacZ expressing cre reporter mouse strains, the progeny is genotyped, and the reporter expression profile characterised in double heterozygous animals. Where relevant, differential activity after tamoxifen dosage is assessed. Other reporters can be used on demand.
The Cre/loxP system provides a valuable method for temporal and tissue-specific modification of the mouse genome. By mating cre driver mouse strains with lacZ expressing cre reporter strains, we are able to reveal cre activity profiles. To enquire about this service, please contact us.
In adult tissues
When relevant, tamoxifen is administrated to double heterozygous cre driver, cre reporter mice and controls. These tamoxifen-treated and non-treated mice are perfused and dissected. The individual tissues are wholemount stained and imaged. We systematically observe and annotate over 40 adult tissues, thereby providing good coverage of the mouse body.
Mice bearing cre driver and reporters are mated, and vaginal plugs recorded. Where relevant, pregnant females are dosed with tamoxifen. Embryos that are the product of the mating of a cre driver and a cre reporter mouse are sacrificed, dissected, fixed and genotyped. These are then either wholemount stained or sectioned and stained, depending on the stage of development, and the gene expression systematically analysed and annotated. To image whole embryos, we use a stereomicroscope or Optical Projection Tomography, and histological sections are scanned using a Nanozoomer platform. We are able to harvest and stain embryos from any requested developmental stage.