The recent emergence of gene editing techniques, such as the CRISPR-Cas9 system, has allowed researchers to quickly produce very specific mouse mutants on highly defined genetic backgrounds, which previous to now would have been expensive both financially and in terms of time. There are many approaches that can be used to generate CRISPR-Cas9 edited mouse models including the use of microinjection technology. This course will teach the use of a cytoplasmic injection technique to generate on-target founder mice for both deletions and point mutations. This technique is relatively simple to learn when compared with the time required to become competent with pronuclear injections.
Participants will have one to one training in embryo harvesting, sorting, set up and operation of their microinjection rig and introducing CRISPR-Cas9 reagents to the embryo using a cytoplasmic injection technique. The course is tailored to the participants ability, with the schedule being flexible to allow more time for injection of one cell embryos if required. For those who are more proficient training in two cell injections, vitrification and non-surgical transfer approaches will be available.
In addition there will be a demonstration of surgical embryo transfer using a surgical assistant to help maintain sterile conditions. There will also be the opportunity to meet and discuss specific issues with experts in IVF technology, CRISPR-Cas9 design or mouse colony management – depending on your specific needs.
More details can be found in the Agenda.