Mouse Embryo and Spermatozoa Cryopreservation
Cryopreservation allows long-term storage of embryos and sperm, providing a convenient way to preserve, protect and transport mouse lines for use in research. Here at MRC Harwell, we have extensive experience of using cryopreservation techniques. This course enables us to share our expertise and help promote resource sharing across the wider scientific community.
This course has been designed to give participants hands-on experience of the entire process from sperm harvesting/freezing, in vitro fertilisation (IVF) recovery and embryo freezing, plus a demonstration of contemporary embryo transfer techniques. The course runs over a period of four days, from 30th March - 2nd April 2020, and is worth ten continuing professional development (CPD) points. It is led by MRC Harwell’s Frozen Embryo and Sperm Archive (FESA), part of the European Mutant Mouse Archive (EMMA), a collective effort by centres all across Europe to create a genetic archive for use by the international scientific community.
Day 1: Participants learn how to freeze sperm in liquid nitrogen using plastic semen straws. While embryos can be stored ready to use, sperm cryopreservation has the advantage that, as long as you have enough oocytes for fertilisation, you can potentially recover over a thousand mice from the sperm of just one male.
Days 2 and 3: Participants will thaw the sperm they froze the previous day and use it to gain experience of a straightforward and robust IVF procedure. Embryos generated by IVF will be frozen by vitrification. At the end of Day 2 everyone has the chance to get to know each other a little better at the course dinner.
Day 4: Participants learn how to thaw the vitrified embryos and will see a demonstration of both surgical and non-surgical embryo transfer techniques. The course concludes with a final review and recap.
More details can be found in the attachment below – Example Cryocourse Agenda.
To see our cryopreservation protocols click here.
The course will be running again from the 30th March to the 2nd April 2020, for more details or to register your interest email: firstname.lastname@example.org