Expression and Cre Activity Studies
Employing lacZ reporter gene
We offer services to characterise the expression pattern of reporter transgenes or lacZ trapping cassettes in genetically altered mice. These can be done either in adults or at various stages of embryonic development.
To request our services, please complete the Expression and Cre Resource Enquiry form.
Cre Activity Profiling
The cre/loxP system provides a valuable method for temporal and tissue-specific modification of the mouse genome. However, a comprehensive characterisation of cre activity is essential for correct interpretation of experimental results.
We offer services in the characterisation of cre driver activity, at both adult and embryonic stages. Cre driver mouse strains are mated with lacZ expressing cre reporter mouse strains, the progeny is genotyped, and the reporter expression profile characterised in double heterozygous animals.
Where relevant, differential activity after tamoxifen dosage can be assessed. Other reporters can be used on demand.
In adult tissues
When relevant, tamoxifen is administrated to double heterozygous cre driver, cre reporter mice and controls. These tamoxifen-treated and non-treated mice are perfused and dissected. The individual tissues are wholemount stained and imaged.
We systematically observe and annotate over 40 adult tissues, to providing a broad survey of the mouse body.
Mice bearing cre driver and reporters are time-mated. Where relevant, pregnant females are dosed with tamoxifen. Embryos are dissected, stained and genotyped.
Embryos are either wholemount stained or sectioned and stained, depending on the stage of development. Reporter gene expression is systematically analysed and annotated. A stereomicroscope or Optical Projection Tomography is employed to image whole embryos. Histological sections are scanned using a Nanozoomer platform.
We offer services to characterise the expression pattern of reporter transgenes or lacZ trapping cassettes in genetically altered mice. These can be performed either in adults or at various stages of embryonic development.
Reporter activity in adult tissues
To study reporter activity in lacZ expressing mice, these mice and controls are sacrificed, perfused and dissected. The separate organs are then whole mount stained and imaged. We systematically observe and annotate the expression patterns of over 40 adult tissues, and therefore provide comprehensive coverage of the body.
Expression profiles are described in the central and peripheral nervous systems, gonads, pituitary glands, adrenal glands, heart, lungs, cartilage, and many other organs and tissues. A stereo-microscope is employed to photograph expressing tissues.
Reporter activity in embryos
LacZ expressing embryos are wholemount stained or sectioned and stained, depending on the stage of development. The expression of the gene of interest is systematically observed and annotated against a list of EMAP terms. Embryos are imaged employing a stereo-microscope or Optical Projection Tomography. Histological sections are scanned using a Nanozoomer to generate pyramidal images.
This service can be used to detect expression in certain areas of interest or as a screen to give an overview of where the reporter gene is expressed.