The cre/loxP system provides a valuable method for temporal and tissue-specific modification of the mouse genome. However, a comprehensive characterisation of cre activity is essential for correct interpretation of experimental results.
We offer services in the characterisation of cre driver activity, at both adult and embryonic stages. Cre driver mouse strains are mated with lacZ expressing cre reporter mouse strains, the progeny is genotyped, and the reporter expression profile characterised in double heterozygous animals.
Where relevant, differential activity after tamoxifen dosage can be assessed. Other reporters can be used on demand.
In adult tissues
When relevant, tamoxifen is administrated to double heterozygous cre driver, cre reporter mice and controls. These tamoxifen-treated and non-treated mice are perfused and dissected. The individual tissues are wholemount stained and imaged.
We systematically observe and annotate over 40 adult tissues, to providing a broad survey of the mouse body.
Mice bearing cre driver and reporters are time-mated. Where relevant, pregnant females are dosed with tamoxifen. Embryos are dissected, stained and genotyped.
Embryos are either wholemount stained or sectioned and stained, depending on the stage of development. Reporter gene expression is systematically analysed and annotated. A stereomicroscope or Optical Projection Tomography is employed to image whole embryos. Histological sections are scanned using a Nanozoomer platform.