We offer services to characterise the expression pattern of reporter transgenes or lacZ trapping cassettes in genetically altered mice. These can be performed either in adults or at various stages of embryonic development.
Reporter activity in adult tissues
To study reporter activity in lacZ expressing mice, these mice and controls are sacrificed, perfused and dissected. The separate organs are then whole mount stained and imaged. We systematically observe and annotate the expression patterns of over 40 adult tissues, and therefore provide comprehensive coverage of the body.
Expression profiles are described in the central and peripheral nervous systems, gonads, pituitary glands, adrenal glands, heart, lungs, cartilage, and many other organs and tissues. A stereo-microscope is employed to photograph expressing tissues.
Reporter activity in embryos
LacZ expressing embryos are wholemount stained or sectioned and stained, depending on the stage of development. The expression of the gene of interest is systematically observed and annotated against a list of EMAP terms. Embryos are imaged employing a stereo-microscope or Optical Projection Tomography. Histological sections are scanned using a Nanozoomer to generate pyramidal images.
This service can be used to detect expression in certain areas of interest or as a screen to give an overview of where the reporter gene is expressed.