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Typically, a genomic sequence is targeted in one-cell embryos by a Cas9 or Cas9 D10A/ sgRNA complex.

The resulting DNA cut is repaired by the cell machinery through NHEJ, giving rise to a deletion or, if a donor DNA was co-delivered, through homologous recombination. The resulting mice are mated and the targeted mutation is transmitted to offspring.

We have established a range of methods, including the use of oligonucleotides or longer single stranded DNA donors and vary reagent delivery method (electroporation, microinjection in the pronecleus or the cytoplasm) for best efficiency according to the desired alleles.

We deliver extensively validated alleles on a variety of genetic backgrounds according to the needs of service users. We assess animals for additional random integration when a donor template is employed.

To request our services, please complete the Mouse model generation Resource Enquiry form.