ADVANCE training centre

Location

ADVANCE training centre
MRC Harwell

Organizer

The Advance Team
Phone
+44 (0)1235 841438
Email
training@har.mrc.ac.uk

Date

21 Mar 2022 - 24 Mar 2022

Time

9:00 am - 2:30 pm

Price

£1,400 per person (VAT exempt)

Microinjection and Electroporation

The Advance mouse genome editing course provides one-to-one tailored and hands-on training in embryo harvesting, set up and operation of microinjection rigs, pronuclear and cytoplasmic microinjection and the process of electroporation to introduce CRISPR-Cas9 reagents to embryos.

In addition, this course offers a demonstration of surgical embryo transfer and the opportunity to meet and discuss your specific requirements with experts in IVF technology, CRISPR-Cas9 design and mouse colony management.

Who is this for?

  • Researchers
  • Senior animal technicians
  • PhD students

Following this course, you will have: 

  • Experience harvesting and sorting 1-cell and 2-cell embryos
  • An understanding of the use of cryopreserved embryos and IVF embryos in gene delivery
  • Practical training in pronuclear and cytoplasmic injection of CRISPR-Cas9 reagent into the embryo
  • Practical training of embryo electroporation
  • An understanding of the principles of colony management, IVF and cryopreservation

Hourly Schedule

Day 1

9:00 - 10:00
Introductions/ Session 1
Registration, Orientation, Coffee, Icebreaker and H&S
10:00 - 12:00
Session 2
1-cell embryo harvesting and sorting (demonstration and practical)
12:00 - 13:00
Lunch
Lunch is provided
13:00 - 13:30
Session 3
1-cell pronuclear and cytoplasmic injection demo (including set up)
13:30 - 15:30
Session 4
1-cell pronuclear and cytoplasmic injection practical
15:30 - 16:00
Session 5
Pulling glass needles and pipettes (bought v's in-house)

Day 2

09:00 - 10:00
Session 1
Tour of the Mary Lyon Centre
10:00 - 12:00
Session 2
2-cell embryo harvesting and sorting (demonstration and practical)
12:00 - 13:00
Lunch
Lunch is provided
13:00 - 13:30
Session 3
2-cell pronuclear and cytoplasmic injection demonstration (incl set up)
13:30 - 15:30
Session 4
2-cell pronuclear and cytoplasmic injection practical
15:30 - 16:00
Session 5
Mix preparation

Day 3

09:00 - 10:00
Session 1
IVF and cryopreservation technology
10:00 - 12:00
Session 2
IVF embryos and electroporation (demonstration and practical)
12:00 - 13:00
Lunch
Lunch is provided
13:00 - 13:30
Session 3
overview of microinjection and its application
13:30 - 15:30
Session 4
Vitrification (demonstration and practical)
15:30 - 16:00
Session 5
Gene delivery QC

Day 4

09:00 - 10:00
Session 1
Colony management
10:00 - 12:00
Session 2
Embryo transfer demonstration and suture and 'Gluture' demonstration and practical
12:00 - 13:00
Lunch
Lunch is provided
13:00 - 13:30
Session 3
Genotyping the outcome of microinjection
13:30 - 14:30
Session 4
Questions and close of course
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  • Thanks for having me in this great course!

    8th Oct 2019

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