Mary Lyon Centre

Location

Mary Lyon Centre
Mary Lyon Centre, Harwell Campus, Didcot, OX11 0RD

Organizer

Dona Reddiar
Phone
+44 (0)1235 841018
Email
training@har.mrc.ac.uk

Date

13 Oct 2020 - 15 Oct 2020

Time

8:00 am - 5:00 pm

Price

£1000 + VAT

1 and 2-Cell Cytoplasmic Microinjection

There are many approaches used for the generation of CRISPR-Cas9 edited mouse models, such as microinjection technology. This course will provide 1-to-1 tailored and hands-on training in embryo harvesting, sorting, set up and operation of microinjection rigs and introducing CRISPR-Cas9 reagents to embryos using the cytoplasmic microinjection technique.

There will also be a demonstration of surgical embryo transfer, and the opportunity to meet and discuss your specific requirements with experts in IVF technology, CRISPR-Cas9 design and mouse colony management.

Hourly Schedule

Day 1

08:00 - 08:30
Introductions
Registration, Orientation, Coffee, Icebreaker and H&S
08:30 - 09:00
Seminar
Introduction to embryo development
09:00 - 10:30
Practical session 1
1-cell harvesting
10:30 - 11:00
Tea and coffee break
11:00 - 11:45
Practical session 2
1-cell embryo sorting
11:45 - 13:00
Practical session 3
Set up and operation of microinjection rig
13:00 - 14:00
Lunch
14:00 - 15:30
Practical session 4
1-cell cytoplasmic injection technique
15:30 - 16:00
Practical session 5
CRISPR mix preparation for cytoplasmic injection
16:00 - 17:00
Seminar
Genotyping the outcome of microinjection

Day 2

08:00 - 09:00
Seminar
Cytoplasmic microinjection - overview and application
09:00 - 10:30
Practical session 1
2-cell embryo harvesting
10:30 - 11:00
Tea and coffee break
11:00 - 11:10
Discussion
What is a healthy 2-cell?
11:10 - 12:30
Practical session 2
Recap of microinjection rig setup and a demo of 2-cell embryo cytoplasmic injection technique
12:30 - 13:30
Lunch
13:30 - 15:00
Practical session 3
2-cell embryo cytoplasmic injection technique
15:00 - 16:00
Practical session 4
Non-surgical embryo transfer demo and practical
16:00 - 17:00
Seminar
IVF and Cryopreservation technology
18:30 - 20:00
Course group dinner

Day 3

08:00 - 09:00
MLC tour
09:00 - 10:00
Seminar
Colony management
10:00 - 10:30
Tea and coffee break
10:30 - 12:15
Practical session 1
Cytoplasmic injection technique (1 and/or 2-cell – your preference)
12:15 - 13:15
Lunch
13:15 - 14:30
Practical session 2
Cytoplasmic injection technique (one and/or two cell – your preference)
14:30 - 15:30
Practical session 3
Demonstration of surgical embryo transfer and review
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  • Thanks for having me in this great course!

    8th Oct 2019

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