COVID 19 Update – Advance Training

Unfortunately, due to the ongoing coronavirus pandemic and current UK lockdown we have decided to cancel all Advance courses for the foreseeable future. We are hoping to re-start our training schedule as soon as possible and will ensure you are kept updated of any changes during this period via the website. We will no longer be taking online bookings at this time, however, if you would like to express an interest in any of the courses or services we have to offer then please contact us at training@har.mrc.ac.uk

Mary Lyon Centre

Location

Mary Lyon Centre
Mary Lyon Centre, Harwell Campus, Didcot, OX11 0RD

Organizer

Advance team
Phone
+44 (0)7546310560
Email
training@har.mrc.ac.uk

Please email for future course interest

Date

27 Apr 2021 - 29 Apr 2021

Time

8:00 am - 5:00 pm

Price

£1000 + VAT

1 and 2-Cell Cytoplasmic Microinjection

There are many approaches used for the generation of CRISPR-Cas9 edited mouse models, such as microinjection technology. This course will provide 1-to-1 tailored and hands-on training in embryo harvesting, sorting, set up and operation of microinjection rigs and introducing CRISPR-Cas9 reagents to embryos using the cytoplasmic microinjection technique.

There will also be a demonstration of surgical embryo transfer, and the opportunity to meet and discuss your specific requirements with experts in IVF technology, CRISPR-Cas9 design and mouse colony management.

Hourly Schedule

Day 1

08:00 - 08:30
Introductions
Registration, Orientation, Coffee, Icebreaker and H&S
08:30 - 09:00
Seminar
Introduction to embryo development
09:00 - 10:30
Practical session 1
1-cell harvesting
10:30 - 11:00
Tea and coffee break
11:00 - 11:45
Practical session 2
1-cell embryo sorting
11:45 - 13:00
Practical session 3
Set up and operation of microinjection rig
13:00 - 14:00
Lunch
14:00 - 15:30
Practical session 4
1-cell cytoplasmic injection technique
15:30 - 16:00
Practical session 5
CRISPR mix preparation for cytoplasmic injection
16:00 - 17:00
Seminar
Genotyping the outcome of microinjection

Day 2

08:00 - 09:00
Seminar
Cytoplasmic microinjection - overview and application
09:00 - 10:30
Practical session 1
2-cell embryo harvesting
10:30 - 11:00
Tea and coffee break
11:00 - 11:10
Discussion
What is a healthy 2-cell?
11:10 - 12:30
Practical session 2
Recap of microinjection rig setup and a demo of 2-cell embryo cytoplasmic injection technique
12:30 - 13:30
Lunch
13:30 - 15:00
Practical session 3
2-cell embryo cytoplasmic injection technique
15:00 - 16:00
Practical session 4
Non-surgical embryo transfer demo and practical
16:00 - 17:00
Seminar
IVF and Cryopreservation technology
18:30 - 20:00
Course group dinner

Day 3

08:00 - 09:00
MLC tour
09:00 - 10:00
Seminar
Colony management
10:00 - 10:30
Tea and coffee break
10:30 - 12:15
Practical session 1
Cytoplasmic injection technique (1 and/or 2-cell – your preference)
12:15 - 13:15
Lunch
13:15 - 14:30
Practical session 2
Cytoplasmic injection technique (one and/or two cell – your preference)
14:30 - 15:30
Practical session 3
Demonstration of surgical embryo transfer and review
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  • Thanks for having me in this great course!

    8th Oct 2019

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