Advance

Location

Advance
MRC Harwell Institute

Organizer

Advance team
Phone
+44 (0)7546310560
Email
training@har.mrc.ac.uk

Date

17 Mar 2021 - 18 Mar 2021

Time

10:00 am - 5:00 pm

Price

£320 + VAT

CRISPR Design and Electroporation

Design your own CRISPR course!

We will provide a background to CRISPR genome-editing technology and its applications in scientific research. You can then decide either to have practical CRISPR-Cas9 training in embryo electroporation, or an in-depth tutorial on planning and designing CRISPR-Cas9 experiments.

Following the course, you will have:

  • An understanding of how the most common CRISPR systems in use work
  • A clear understanding of advantages and limitations of genome editing for germline transgenesis
  • An ability to design CRISPR-Cas9 experiments with appropriate quality control strategies that are essential for reproducible and robust research or
  • Practical experience in using CRISPR-Cas9 in embryo electroporation

Hourly Schedule

Day 1 - CRISPR background

10:00 - 10:30
Introductions
Coffee, registration, Housekeeping and H&S
10:30 - 12:30
Session 1
Introduction to Genome Editing, Gene delivery to embryos and ES cells, Workshop 1
12:30 - 13:15
Lunch
13:15 - 14:00
Seminar
Strategies for the generation and validation of complex alleles
14:00 - 14:30
Workshop
Validation of complex alleles
14:30 - 14:50
Tea and coffee break
14:50 - 15:15
Workshop - continued
15:15 - 15:45
Seminar
Genome editing, 3Rs and research reproducibility
15:45 - 16:00
Course recap and review
Final questions
16:00 - 17:00
Tour (optional)
Mary Lyon Centre

Day 2 - CRISPR experimental design

Day 2 - Embryo electroporation

08:00 - 09:00
Seminar
Introduction: ‘Delivery to the Cytoplasm’
09:00 - 10:30
Practical
Embryo harvesting
10:30 - 11:00
Tea and coffee break
11:00 - 11:50
Practical
Embryo sorting
12:00 - 13:00
Demonstration
CRISPR mix preparation
13:00 - 13:45
Lunch
14:00 - 15:00
Practical
Embryo electroporation
15:00 - 16:00
Demonstration
Surgical embryo transfer
16:00 - 16:30
Course recap and review
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  • It’s just perfect!!! Thank you all so much.

    11th Nov 2019

  • Thank you again for the opportunity (and all the questions you answered me), it has been an exciting experience that I cannot wait to establish here.

    11th Nov 2019

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