COVID 19 Update – Advance Training

Unfortunately, due to the ongoing coronavirus pandemic and current UK lockdown we have decided to cancel all Advance courses for the foreseeable future. We are hoping to re-start our training schedule as soon as possible and will ensure you are kept updated of any changes during this period via the website. We will no longer be taking online bookings at this time, however, if you would like to express an interest in any of the courses or services we have to offer then please contact us at training@har.mrc.ac.uk

Advance

Location

Advance
MRC Harwell Institute

Organizer

Advance team
Phone
+44 (0)7546310560
Email
training@har.mrc.ac.uk

Please email for future course interest

Date

17 Mar 2021 - 18 Mar 2021

Time

10:00 am - 5:00 pm

Price

£320 + VAT

CRISPR Design and Electroporation

Design your own CRISPR course!

We will provide a background to CRISPR genome-editing technology and its applications in scientific research. You can then decide either to have practical CRISPR-Cas9 training in embryo electroporation, or an in-depth tutorial on planning and designing CRISPR-Cas9 experiments.

Following the course, you will have:

  • An understanding of how the most common CRISPR systems in use work
  • A clear understanding of advantages and limitations of genome editing for germline transgenesis
  • An ability to design CRISPR-Cas9 experiments with appropriate quality control strategies that are essential for reproducible and robust research or
  • Practical experience in using CRISPR-Cas9 in embryo electroporation

Hourly Schedule

Day 1 - CRISPR background

10:00 - 10:30
Introductions
Coffee, registration, Housekeeping and H&S
10:30 - 12:30
Session 1
Introduction to Genome Editing, Gene delivery to embryos and ES cells, Workshop 1
12:30 - 13:15
Lunch
13:15 - 14:00
Seminar
Strategies for the generation and validation of complex alleles
14:00 - 14:30
Workshop
Validation of complex alleles
14:30 - 14:50
Tea and coffee break
14:50 - 15:15
Workshop - continued
15:15 - 15:45
Seminar
Genome editing, 3Rs and research reproducibility
15:45 - 16:00
Course recap and review
Final questions
16:00 - 17:00
Tour (optional)
Mary Lyon Centre

Day 2 - CRISPR experimental design

Day 2 - Embryo electroporation

08:00 - 09:00
Seminar
Introduction: ‘Delivery to the Cytoplasm’
09:00 - 10:30
Practical
Embryo harvesting
10:30 - 11:00
Tea and coffee break
11:00 - 11:50
Practical
Embryo sorting
12:00 - 13:00
Demonstration
CRISPR mix preparation
13:00 - 13:45
Lunch
14:00 - 15:00
Practical
Embryo electroporation
15:00 - 16:00
Demonstration
Surgical embryo transfer
16:00 - 16:30
Course recap and review

The event is finished.

  • It’s just perfect!!! Thank you all so much.

    11th Nov 2019

  • Thank you again for the opportunity (and all the questions you answered me), it has been an exciting experience that I cannot wait to establish here.

    11th Nov 2019

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