Complex Genetically Altered Mice

For: Colony Managers and Researchers

This course will cover more complex genetics including multiple allele crosses and will inform breeding strategies and experimental design rather than give a complete guide to the molecular biology. We will examine how to plan breeding schemes, how to identify appropriate controls and analyse recombinase (Cre) expression.

After the course, you will be able to:

• Predict the outcomes of genetic crosses
• Understand the fundamentals of mouse genetic engineering
• Have a working knowledge of the genetic background of mouse strains
• Understand how to maintain the genetic integrity of inbred lines
• Perform multiple allele inheritance calculations
• Understand how Genetically Altered lines can be established from modern transgenic technologies
• An understanding of the basic principles of conditional mutagenesis
• Practical experience in setting up breeding programmes for conditional mutagenesis, including the choice of appropriate control cohorts