Location
- MRC Harwell
Fully booked
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Microinjection and Electroporation
The Microinjection and Electroporation course provides tailored and hands-on training in embryo harvesting, set up and operation of microinjection rigs, pronuclear and cytoplasmic microinjection and the process of electroporation to introduce CRISPR-Cas9 reagents to embryos.
In addition, this course offers a demonstration of surgical embryo transfer and the opportunity to meet and discuss your specific requirements with experts in IVF technology, CRISPR-Cas9 design and mouse colony management.
Who is this for?
- Researchers
- Senior animal technicians
- PhD students
Following this course, you will have:
- Experience harvesting and sorting 1-cell and 2-cell embryos
- An understanding of the use of cryopreserved embryos and IVF embryos in gene delivery
- Practical training in pronuclear and cytoplasmic injection of CRISPR-Cas9 reagent into the embryo
- Practical training of embryo electroporation
- An understanding of the principles of colony management, IVF and cryopreservation
Hourly Schedule
Day 1
- 9:00 - 10:00
- Introductions/ Session 1
- Registration, Orientation, Coffee, Icebreaker and H&S
- 10:00 - 12:00
- Session 2
- 1-cell embryo harvesting and sorting (demonstration and practical)
- 12:00 - 13:00
- Lunch
- Lunch is provided
- 13:00 - 13:30
- Session 3
- 1-cell pronuclear and cytoplasmic injection demo (including set up)
- 13:30 - 15:30
- Session 4
- 1-cell pronuclear and cytoplasmic injection practical
- 15:30 - 16:00
- Session 5
- Pulling glass needles and pipettes (bought v's in-house)
Day 2
- 09:00 - 10:00
- Session 1
- Tour of the Mary Lyon Centre
- 10:00 - 12:00
- Session 2
- 2-cell embryo harvesting and sorting (demonstration and practical)
- 12:00 - 13:00
- Lunch
- Lunch is provided
- 13:00 - 13:30
- Session 3
- 2-cell pronuclear and cytoplasmic injection demonstration (incl set up)
- 13:30 - 15:30
- Session 4
- 2-cell pronuclear and cytoplasmic injection practical
- 15:30 - 16:00
- Session 5
- Mix preparation
Day 3
- 09:00 - 10:00
- Session 1
- IVF and cryopreservation technology
- 10:00 - 12:00
- Session 2
- IVF embryos and electroporation (demonstration and practical)
- 12:00 - 13:00
- Lunch
- Lunch is provided
- 13:00 - 13:30
- Session 3
- Overview of microinjection and its application
- 13:30 - 15:30
- Session 4
- Vitrification (demonstration and practical)
- 15:30 - 16:00
- Session 5
- Gene delivery QC
Day 4
- 09:00 - 10:00
- Session 1
- Colony management
- 10:00 - 12:00
- Session 2
- Embryo transfer demonstration and suture and 'Gluture' demonstration and practical
- 12:00 - 13:00
- Lunch
- Lunch is provided
- 13:00 - 13:30
- Session 3
- Genotyping the outcome of microinjection
- 13:30 - 14:30
- Session 4
- Questions and close of course
