Microinjection and Electroporation

The Microinjection and Electroporation course provides tailored and hands-on training in embryo harvesting, set up and operation of microinjection rigs, pronuclear and cytoplasmic microinjection and the process of electroporation to introduce CRISPR-Cas9 reagents to embryos.

In addition, this course offers a demonstration of surgical embryo transfer and the opportunity to meet and discuss your specific requirements with experts in IVF technology, CRISPR-Cas9 design and mouse colony management.

Who is this for?

• Researchers
• Senior animal technicians
• PhD students

Following this course, you will have: 

• Experience harvesting and sorting 1-cell and 2-cell embryos
• An understanding of the use of cryopreserved embryos and IVF embryos in gene delivery
• Practical training in pronuclear and cytoplasmic injection of CRISPR-Cas9 reagent into the embryo
• Practical training of embryo electroporation
• An understanding of the principles of colony management, IVF and cryopreservation