Advance

Location

Advance
MRC Harwell Institute

Organizer

Dona Reddiar
Phone
+44 (0)1235 841018
Email
training@har.mrc.ac.uk

Date

19 Oct 2020 - 22 Oct 2020

Time

8:30 am - 4:30 pm

Price

£1100 + VAT

Mouse Embryo and Spermatozoa Cryopreservation

Cryopreservation allows long-term storage of embryos and sperm, providing a convenient way to preserve, protect and transport mouse lines for use in research. This course provides practical experience in murine embryo and spermatozoa freezing techniques routinely used at MRC Harwell and a simple, robust in vitro fertilisation procedure.

Running bi-annually since 2004, this course is led by MRC Harwell’s Frozen Embryo and Sperm Archive (FESA), part of the European Mutant Mouse Archive (EMMA), a collective effort by centres all across Europe to create a genetic archive for use by the international scientific community.

Hourly Schedule

Day 1

08:30 - 09:00
Registration
09:00 - 10:00
Introduction
Lecture, coffee and discussion
10:00 - 11:00
MLC Tour
11:00 - 12:00
Practical session 1
Pipetting, weighing, microscopy use, handling embryos
12:00 - 13:00
Lunch
13:00 - 15:00
Practical session 2
Sperm Harvesting and Freezing Introduction
15:00 - 16:00
Practical session 3
Preparation work for Day 2 IVF
16:00 - 16:30
Recap and review

Day 2

07:30 - 10:30
Practical session 1
IVF introduction
10:30 - 11:00
Tea and coffee break
11:00 - 12:30
Optional
Tour of Microinjection lab or embryo harvest
12:30 - 13:30
Lunch
13:30 - 15:00
Practical session 2
Wash IVF
15:00 - 16:00
Seminar
Handling and shipping samples introduction
16:00 - 16:30
Recap and review
19:30 - 21:30
Course group dinner

Day 3

08:30 - 09:30
Practical session 1
Score IVF
09:30 - 10:00
Practical session 2
Embryo Vitrification demonstration
10:00 - 10:30
Tea and coffee break
10:30 - 11:30
Practical session 3
Embryo freezing – vitrification
11:30 - 12:30
Practical session 4
Thawing embryos
12:30 - 13:30
Lunch
13:30 - 14:30
Practical session 5
Media preparation, pulling pipettes, blastocyst genotyping
14:30 - 15:30
Workshop
Managing the Archive
15:30 - 16:00
Recap and review

Day 4

08:30 - 09:00
Practical session 1
Controlled rate embryo freezing
09:00 - 09:30
Practical session 2
Thawing embryos from controlled rate freezing method
09:30 - 10:30
Practical session 3
Non- Surgical Embryo Transfer – NSET/TCET Subcuticular Stitch and Wound Glue
10:30 - 11:00
Tea and coffee break
11:00 - 12:00
Practical session
Surgical embryo transfer demonstration
12:00 - 12:30
Recap and review
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  • A massive thanks to you and all your team for the fantastic training course! Have definitely picked up lots of tips which I'll be implementing (especially the subcuticular stitch).

    23rd Sep 2019

  • I would just like to say what fantastic course, I'm still processing some of the information provided and have forward some ideas on to my manager so hopeful we may be putting a few new things in place and updating the old ones. A big thank you to you and your staff.

    23rd Sep 2019

  • I wanted to thank you for this professional and fantastic training course! All Staff were very nice and with great knowledge. I wish to find the staff as in MRC and I know it will not be easy...I was very happy to be a part of this course! I picked up a lot of tips and I’m sure it will promote my IVF lab work.

    23rd Sep 2019

  • Really enjoyed the course, thought the information was well presented and clear. Great ratio of staff to trainees.

    23rd Sep 2019

  • Very indepth course, well laid out. Group perfect size, anymore would be too crowded. Questions answered or found out from someone. Great to see development of new techniques and methods.

    23rd Sep 2019

  • Thank you all for a great week. We all agree that this has been the best, most productive course we’ve been on. The company has been brilliant and we hope we will all be able to keep in touch in the future.

    25th Mar 2019

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