Advance

Location

Advance
MRC Harwell Institute

Organizer

The Advance Team
Phone
+44 (0)1235 841438
Email
training@har.mrc.ac.uk

Date

28 Mar 2022 - 31 Mar 2022

Time

8:30 am - 3:30 pm

Price

£1,400 per person (VAT exempt)

Mouse Embryo and Spermatozoa Cryopreservation

Cryopreservation allows long-term storage of embryos and sperm, providing a convenient way to preserve, protect and transport mouse lines for use in research. Archiving scientifically relevant mouse lines also adheres to good animal welfare practices by removing the need for continued animal breeding and is integral to any comprehensive colony management programme.

Running bi-annually since 2004, this course provides practical experience in murine embryo and spermatozoa freezing techniques routinely used at MRC Harwell and a simple, robust in vitro fertilisation procedure. The course will also cover the basic laboratory skills required when working in an assisted reproductive biology environment such as pipetting, weighing, microscope use, and embryo handling.

Who is this for? 

  • Researchers
  • Senior animal technicians
  • PhD students

Following this course, you will have:

  • Practical experience of sperm harvesting and freezing/thawing
  • A clear understanding to set up and in vitro fertilisation session using both frozen and freshly harvested sperm
  • Practical experience of oocyte and embryo harvesting for in vitro fertilisation and embryo freezing, respectively
  • Practical experience of embryo cryopreservation/thawing using a simple vitrification method
  • An understanding of surgical embryo transfer, including some practical experience of pre-operative aseptic techniqies and sub-cuticular suturing
  • Practical experience of non-surgical embryo transfer techniques
  • An understanding of the current methods used for handling and shipping samples and managing a cryo-archive, as well as an overview of blastocyst genotyping

 

Hourly Schedule

Day 1

08:30 - 09:00
Registration
Arrival and registration
09:00 - 10:00
Introductory lecture
Lecture, coffee and discussion
10:00 - 11:00
MLC Tour
11:00 - 12:00
Practical session 1
Pipetting, weighing, microscopy use, handling embryos
12:00 - 13:00
Lunch
13:00 - 15:00
Practical session 2
Sperm Harvesting and Freezing Introduction
15:00 - 16:00
Practical session 3
Preparation work for Day 2 IVF
16:00 - 16:30
Recap and review

Day 2

07:30 - 10:30
Practical session 1
IVF introduction
10:30 - 11:00
Tea and coffee break
11:00 - 12:30
Optional
Tour of Microinjection lab or embryo harvest
12:30 - 13:30
Lunch
13:30 - 15:00
Practical session 2
Wash IVF
15:00 - 16:00
Seminar
Handling and shipping samples introduction
16:00 - 16:30
Recap and review
19:30 - 21:30
Course group dinner

Day 3

08:30 - 09:30
Practical session 1
Score IVF
09:30 - 10:00
Practical session 2
Embryo Vitrification demonstration
10:00 - 10:30
Tea and coffee break
10:30 - 11:30
Practical session 3
Embryo freezing – vitrification
11:30 - 12:30
Practical session 4
Thawing embryos
12:30 - 13:30
Lunch
13:30 - 14:30
Practical session 5
Media preparation, pulling pipettes, blastocyst genotyping
14:30 - 15:30
Workshop
Managing the Archive
15:30 - 16:00
Recap and review

Day 4

08:30 - 09:00
Practical session 1
Controlled rate embryo freezing
09:00 - 09:30
Practical session 2
Thawing embryos from controlled rate freezing method
09:30 - 10:30
Practical session 3
Non- Surgical Embryo Transfer – NSET/TCET Subcuticular Stitch and Wound Glue
10:30 - 11:00
Tea and coffee break
11:00 - 12:00
Practical session
Surgical embryo transfer demonstration
12:00 - 12:30
Recap and review
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  • A massive thanks to you and all your team for the fantastic training course! Have definitely picked up lots of tips which I'll be implementing (especially the subcuticular stitch).

    23rd Sep 2019

  • I would just like to say what fantastic course, I'm still processing some of the information provided and have forward some ideas on to my manager so hopeful we may be putting a few new things in place and updating the old ones. A big thank you to you and your staff.

    23rd Sep 2019

  • I wanted to thank you for this professional and fantastic training course! All Staff were very nice and with great knowledge. I wish to find the staff as in MRC and I know it will not be easy...I was very happy to be a part of this course! I picked up a lot of tips and I’m sure it will promote my IVF lab work.

    23rd Sep 2019

  • Really enjoyed the course, thought the information was well presented and clear. Great ratio of staff to trainees.

    23rd Sep 2019

  • Very indepth course, well laid out. Group perfect size, anymore would be too crowded. Questions answered or found out from someone. Great to see development of new techniques and methods.

    23rd Sep 2019

  • Thank you all for a great week. We all agree that this has been the best, most productive course we’ve been on. The company has been brilliant and we hope we will all be able to keep in touch in the future.

    25th Mar 2019

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